Rahul Singh Parmar/LinkedIn
Aug 28, 2026, 13:21
Rahul Singh Parmar։ IVF Lab QC – Morphological Sperm Selection and DFI Management
Rahul Singh Parmar, Senior Clinical Embryologist at Indira IVF Group, shared on LinkedIn։
“IVF Lab QC: Morphological Sperm Selection and DFI Management․
Selecting high-quality sperm is crucial for successful fertilization and blastocyst development in ICSI cycles. Standard sperm preparation methods often fall short, particularly in cases with a high Sperm DNA Fragmentation Index (DFI).
Sperm Selection Methods: A QC Comparison
- Density Gradient Centrifugation (DGC): Isolates highly motile sperm, but high-speed centrifugation carries a risk of generating Reactive Oxygen Species (ROS).
- Swim-Up Technique: Delivers a clean yield for low-DFI samples, but recovery rates drop significantly in cases of severe oligo-asthenozoospermia.
- Microfluidic Sperm Sorting (Chip Method): A single-step, non-centrifugation process that minimizes double-stranded DNA damage, leading to superior blastocyst conversion rates in high-DFI cases.
- IMSI / Physiological Selection (PICS): Utilizes hyaluronic acid binding or 6000× magnification to select mature, vacuole-free sperm.
Key QC Considerations for Embryologists
- Centrifugation Speed and Time: Avoid excessive force (>300g) to prevent sperm membrane damage and oxidative stress.
- Holding Temperature: Maintain prepared sperm samples at optimal temperatures to prevent a decline in motility.
What is your laboratory’s primary SOP for sperm preparation in high-DFI cases?”
Stay informed with the latest updates in fertility and reproductive medicine on Fertility News.
-
Oct 18, 2026, 18:01Ting Yuan: Attending a Locally Organised Reproductive Medicine Conference in Malaysia with My Team
-
Oct 18, 2026, 17:55Khalil Faaed: Ovarian Rejuvenation with PRP
-
Oct 18, 2026, 17:49UCLA Law Center on Reproductive Health, Law, and Policy Hosts Panel on Menopause and Women’s Health Equity
-
Oct 18, 2026, 17:41Arora Suhani Gagan: Everyone Was Discussing Her Pregnancy, but Nobody Asked What She Wanted
-
Oct 18, 2026, 17:07Robert Borowczyk: Fertility Questions Need Different Answers
-
Oct 18, 2026, 17:05Farah Yasin: Another Successful ART Fertility Clinics Conference in Abu Dhabi
-
Oct 18, 2026, 17:01Hilary Friedlander: Excited to Offer NeedleFree IVF at Atlantic Fertility
-
Oct 18, 2026, 16:59Rabih Chaoui: New Edition of “First Trimester Ultrasound Diagnosis of Fetal Abnormalities”
-
Oct 18, 2026, 16:56Arsiak Vartenian: My Daughter Was Born Through Surrogacy, and That Is a Story Worth Telling