Khaled Abu Ali: Can We Warm Vitrified Blastocysts in a Single Step?
Khaled Abu Ali, IVF Unit Director at the American Hospital Dubai, shared on LinkedIn:
“Can we warm vitrified blastocysts in a single step, and does the sucrose concentration matter?
That was the question behind the poster I presented at ESHRE 2023, together with Aisling Ahlström, whose guidance and generosity with her knowledge shaped so much of my work in the lab. Thank you, Aisling.
Most warming protocols still use three to five steps with decreasing sucrose concentrations.
Each extra step adds handling tim, and time outside the incubator.
We tested single-step warming at 37°C using 0.25 M, 0.5 M and 1.0 M sucrose.
Overall, 98% of blastocysts were transferable after warming and 96% remained viable after 24 hours of culture, with no difference between concentrations.
The takeaway for the lab: fewer steps, less exposure, simpler workflow, without compromising survival.
Simplifying a protocol is often harder than adding to it.
It needs evidence before anyone trusts it.”
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