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Youhe Gao: Insights on Pregnancy Through Urine Proteomics

Youhe Gao/preprints.org

Dec 2, 2025, 14:04

Youhe Gao: Insights on Pregnancy Through Urine Proteomics

Youhe Gao, Professor at Beijing Normal University, shared a post on LinkedIn about a paper published in Biology:

“AI reads Urine

Proteome Analysis of Daily Urine Samples of Pregnant Rats Unveils Developmental Processes of Fetus as Well as Physiological Changes in Mother Rats

To explore the dynamic physiological changes of the mother and fetus during pregnancy (given the limitations of directly studying human pregnancy, rats were selected as experimental subjects), this study collected daily urine samples from 4 rats in the pregnancy group (from before mating to day 18 of pregnancy) and 4 rats in the control group. It analyzed the differences in urinary proteins using magnetic nanobeads for low-abundance protein enrichment and proteomic techniques (such as liquid chromatography-tandem mass spectrometry, LC-MS/MS), and identified a total of 3455 types of urinary proteins. The study found that changes in urinary proteins in the early stage of pregnancy reflected blastocyst formation and cell division; in the middle stage (especially on day 9 of pregnancy), they concentratedly indicated the development of organs such as the nervous system and vascular system; in the late stage, maternal-specific changes appeared, including those related to lactation and adjustments in blood coagulation function. Additionally, urinary proteins could clearly reflect fetal development and the mother’s physiological adaptation process during pregnancy. This research provides a new basis for understanding the maternal physiological changes during pregnancy, exploring the mechanisms of pregnancy-related diseases in humans, and developing pregnancy monitoring indicators. However, it has limitations: the sample size of each group is only 4 rats (resulting in limited statistical power), and further verification in humans is required.

Biology 2025, 14(12), 1700.

Youhe Gao

With Doubao

2025.11.29

For earlier AI Reads Urine articles.”

Title: Proteome Analysis of Daily Urine Samples of Pregnant Rats Unveils Developmental Processes of Fetus as Well as Physiological Changes in Mother Rats

Authors: Haitong Wang, Linna Ge, Sijie Chen, Longqin Sun, Wei Sun, Youhe Gao

Read the full article.

Youhe Gao