Rahul Manoj: Sperm Preparation Techniques for IVF and ICSI
Rahul Manoj, Senior Embryologist at Best Life Fertility Center, shared a post on LinkedIn:
“Day 12 – Sperm Preparation Techniques for IVF and ICSI
Efficient sperm preparation is crucial for isolating motile, morphologically normal, and DNA-intact spermatozoa — essential for fertilization success in both IVF and ICSI.
The choice of technique depends on semen parameters and the clinical scenario.
Major Techniques:
- Swim-Up Method/direct layering
• Relies on sperm motility for separation.
• Best suited for normozoospermic samples. - Density Gradient Centrifugation (DGC)
• Separates sperm based on density and morphology.
• Effective for samples with debris or low motility. - Simple Wash Method
• Removes seminal plasma quickly.
• Common in donor sample processing. - Microfluidic Sperm Sorting
• Mimics natural sperm selection in the female tract.
• Reduces oxidative stress and improves DNA integrity.
Quality Control Considerations:
Maintain sperm viability and motility throughout the procedure.
Control centrifugation force, temperature, and pH.
Routinely check media osmolality and sterility.
lets Discuss:
> Between Density Gradient Centrifugation and Microfluidic Sperm Sorting, which do you believe offers better DNA integrity and improved blastocyst development potential — and why?
The image is taken from the paper below and also refer to this paper for more information on semen washing techniques.”
-
Apr 15, 2026, 16:38Why an Ovulation Trigger Shot Sometimes Fails – Fertility Plus
-
Apr 15, 2026, 16:3374 New Studies Added to the AC Forum Literature Library – Anticoagulation Forum
-
Apr 15, 2026, 16:23Insights from PGT-A in Recurrent IVF Failure – Motherhood Training Center
-
Apr 15, 2026, 16:20Effect of Endometriosis and Adenomyosis on Oocyte Quality and Morphology – RBMO
-
Apr 15, 2026, 11:37Ally Murji: Honoured to be Part of the Canadian Session at the SEUD Meeting
-
Apr 15, 2026, 11:00Scammers are Targeting ESHRE2026 Attendees With Fake Registration and Hotel Offers – ESHRE
-
Apr 15, 2026, 10:24Join Us This Friday for Our “Research in Progress” – SRF
-
Apr 15, 2026, 10:20The Importance of Fertility Preservation in Turner Syndrome – RBMO
-
Apr 15, 2026, 10:16Marlena Fejzo: Our Study Identifying 6 New Genes Associated with Hyperemesis Gravidarum