Oct 31, 2025, 14:35
Training Future Andrologists at Conceive Research and Art Bank – Conceive Research
Conceive Research shared a post on LinkedIn:
“Mastering DFI – one sample at a time!
Training future andrologists at CONCEIVE Research and Art Bank.
The Sperm Chromatin Dispersion (SCD) or Halo Test is a direct method used to assess the integrity of sperm DNA by observing the presence and size of a “halo” around the sperm head.
Key Principle
- Sperm with intact (non-fragmented) DNA will produce a large, diffuse loop of DNA that forms a distinct, bright halo surrounding the sperm head nucleus when treated with an acid and lysis solution.
- Sperm with fragmented (damaged) DNA lack the structural integrity to form these large loops, resulting in a small, poorly defined, or absent halo.
Clinical Significance
A high DFI (>30%) is associated with:
- Lower fertilization rates (in some cases).
- Poorer embryo quality and development.
- Increased risk of miscarriage (recurrent pregnancy loss).
- Failure of IVF/ICSI cycles.”
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